The Fabrication of a Gellan Gum-Based Hydrogel Loaded With Magnesium Ions for the Synergistic Promotion of Skin Wound Healing
Wenqiang Li, Xingling Jian, Yanfen Zou, Lin Wu, Haiyan Huang, Hui Li, Dandan Hu, Bo Yu
Abstract
To accelerate serious skin burn wound healing in a convenient manner, an interpenetrating network of hydrogel consisting of gellan gum and polyacrylamide was synthesized by chemical crosslinking and Mg 2+ ion immersion techniques. The prepared Mg 2+ @PAM/GG hydrogel was characterized by morphology, water vapor loss, swelling ratio, rheological properties, tensile mechanical, biocompatibility, and flow cytometry study. The results show that Mg 2+ @PAM/GG hydrogel’s mechanical strength could be enhanced by the dual network structure and physical crosslinking agent Mg 2+ ions. In addition, the tension strength of Mg 2+ @PAM/GG hydrogel is obviously increased from 86 to 392 kPa, the elongation at break increased from 84 to 231%, and crosslinking density N increased from 4.3 to 7.2 mol/m 3 compared with pure GG hydrogel. The cumulative release curve of Mg 2+ ions shows that the multiple release mechanism of Mg 2+ ions belong to non-Fick’s diffusion. Meanwhile, in vitro experiments show that Mg 2+ @PAM/GG double network hydrogel has favorable proliferation and an NF-κB pathway inhibition property for fibroblast cells. Finally, the healing effect of the Mg 2+ @PAM/GG was evaluated in a rat full-thickness burn model. The animal study demonstrates that Mg 2+ @PAM/GG could accelerate the healing efficiency in case of the sustained-released Mg 2+ ions in wound beds. Considering this excellent performance, this convenient prepared hydrogel has great potential as a commercial application for skin full-thickness burn healing materials.