Preparative production of an enantiomeric pair by engineered polyketide synthases
Takeshi Miyazawa, Brendan J. Fitzgerald, Adrian T. Keatinge‐Clay
Abstract
Using the updated module boundary of polyketide assembly lines, modules from the pikromycin synthase were recombined into engineered synthases that furnish an enantiomeric pair of 2-stereocenter triketide lactones at >99% ee with yields up to 0.39 g per liter of E. coli K207-3 in shake flasks.
Topics & Concepts
PolyketideChemistryEnantiomerStereochemistryCombinatorial chemistryBiosynthesisBiochemistryEnzymeEnzyme Catalysis and ImmobilizationMicrobial Natural Products and BiosynthesisMicrobial Metabolic Engineering and Bioproduction