Peptide-mediated delivery of CRISPR enzymes for the efficient editing of primary human lymphocytes
Dana V. Foss, Joseph J. Muldoon, David N. Nguyen, Daniel J.J. Carr, Srishti U. Sahu, John M. Hunsinger, Stacia K. Wyman, Netravathi Krishnappa, Rima Mendonsa, Elaine V. Schanzer, Brian R. Shy, Vivasvan S. Vykunta, Vincent Allain, Zhongmei Li, Alexander Marson, Justin Eyquem, Ross C. Wilson
Topics & Concepts
CRISPRRibonucleoproteinGenome editingElectroporationTrans-activating crRNABiologyGenome engineeringMolecular biologyGene deliveryCas9Homology directed repairTransfectionPeptideGuide RNACell biologyComputational biologyGeneRNAGeneticsBiochemistryDNA repairDNA mismatch repairCRISPR and Genetic EngineeringCAR-T cell therapy researchCytomegalovirus and herpesvirus research