Establishment of portable Pseudomonas aeruginosa detection platform based on one-tube CRISPR/Cas12a combined with recombinase polymerase amplification technology
Haitao Yang, Aibo Liu, Fenfen Ma, Xuzhu Gao, Kun Wang, Yan Wang, Yan Wang
Topics & Concepts
CRISPRRecombinase Polymerase AmplificationTrans-activating crRNAPseudomonas aeruginosaPrimer (cosmetics)BiologyComputational biologyGenotypingAmpliconMicrobiologyLoop-mediated isothermal amplificationPolymerase chain reactionBacteriaGeneticsChemistryGeneDNACas9GenotypeOrganic chemistryCRISPR and Genetic EngineeringBiosensors and Analytical DetectionBacterial biofilms and quorum sensing