An improvement of the 2ˆ(-delta delta CT) method for quantitative real-time polymerase chain reaction data analysis.
Xiayu Rao, Xuelin Huang, Zhicheng Zhou, Xin Lin
Abstract
BACKGROUND: method is applied to data with automatic removal of background fluorescence by the qPCR software. Since the background fluorescence is unknown, subtracting an inaccurate background can lead to distortion of the results. To address these problems, we present an improved method, the individual efficiency corrected calculation. RESULTS: method. CONCLUSIONS: method and is thus a better way to calculate relative gene expression.
Topics & Concepts
Background subtractionReal-time polymerase chain reactionSubtractionPolymerase chain reactionBiological systemSample (material)Computer scienceMathematicsGeneComputational biologyBiologyChemistryChromatographyArtificial intelligenceGeneticsPixelArithmeticMolecular Biology Techniques and ApplicationsGene expression and cancer classificationRNA Research and Splicing