PI3K/Akt pathway expression in children with different obesity degrees and its relationship with glucolipid metabolism and insulin resistance.
Xiaoyan Su, Deming Gu, Xu Li, Zhenming Liang, Xuan Luo, Yang Pu, Jing Yang
Abstract
OBJECTIVE: This study investigated and analyzed the expression of PI3K/Akt pathway in children with different degree of obesity and its connection with glucolipid metabolism and insulin resistance (IR). METHODS: 157 children with simple obesity, who admitted to our hospital from March 2020 to September 2020, were enrolled as obesity group. These children were divided into mild-group (n=67), moderate-group (n=55) and severe-group (n=35) referring to their body mass index (BMI). Another 60 healthy children admitted to hospitalized were randomly chosen as control group. The expression of PI3K mRNA and Akt mRNA in peripheral blood mononuclear cells (PBMCs) of each group were detected by RT-PCR, and its connection with glucose and lipid metabolism, as well as IR was analyzed. RESULTS: ). CONCLUSION: The inhibition of PI3K/Akt signaling pathway in children with simple obesity is associated with the abnormal glucolipid metabolism and IR, which affects the occurrence and progression of obesity.