Update on Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Identification of Filamentous Fungi
Laura S. Wilkendorf, Edmee C. Bowles, Jochem B. Buil, Henrich A. L. van der Lee, Brunella Posteraro, Maurizio Sanguinetti, Paul E. Verweij
Abstract
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based species identification has found its place in many clinical routine diagnostic laboratories over the past years, allowing significantly reduced turnaround times and high-precision results. With regard to MALDI-TOF MS for filamentous fungi, here, we discuss different approaches for sample processing and growth conditions before analysis. In particular, we review the performances of different commercially available databases as well as the potential of complementary (self-constructed) in-house databases.
Topics & Concepts
Mass spectrometryDesorptionIdentification (biology)ChromatographyMatrix-assisted laser desorption/ionizationClinical microbiologyMatrix (chemical analysis)Time-of-flight mass spectrometryIonizationAnalytical Chemistry (journal)ChemistryBiologyMicrobiologyBotanyIonOrganic chemistryAdsorptionBacterial Identification and Susceptibility TestingPlant Pathogens and Fungal DiseasesGenomics and Phylogenetic Studies