Toxicological Risks of Renqingchangjue in Rats Evaluated by <sup>1</sup>H NMR-Based Serum and Urine Metabolomics Analysis
Xia Wang, Caidan Rezeng, Yingfeng Wang, Jian Li, Lan Zhang, Jianxin Chen, Zhong‐Feng Li
Abstract
H NMR-based metabolomics analysis revealed that the administration of RQCJ significantly altered the concentrations of 14 serum metabolites and 14 urine metabolites, which implied disturbances in energy metabolism, amino acid metabolism, intestinal flora environment, and membrane damage. Besides, the biochemical analysis of serum samples was consistent with the histopathological results, which indicated slight hepatotoxicity and nephrotoxicity. The quantification of As and Hg in urine and serum samples by ICP-MS provided more evidence about the toxicity of RQCJ. This work provided an effective method to systematically and dynamically evaluate the toxicity of RQCJ and suggested that precautions should be taken in the clinic to monitor the potential toxicity of RQCJ.