Advances and prospects in the biosynthesis of vitamin B<sub>6</sub> and its bioengineering as a cofactor of other chemicals
Lijuan Wu, Linxia Liu, Zhaoxia Jin, Dawei Zhang
Abstract
Abstract Vitamin B 6 is an essential nutrient, which is widely used in food products, feed additives, pharmaceuticals, disease diagnosis, and other fields. Pyridoxal‐5′‐phosphate (PLP), the active form of vitamin B 6 , is an important coenzyme participating in a variety of enzyme reactions. At present, the oxazole method is mainly used for the production of vitamin B 6 , but toxic and corrosive intermediates produced in the synthesis process, which does not conform to the green manufacturing. Therefore, there is considerable interest in shifting from chemical processes to sustainable fermentation techniques and research on PLP metabolism of other valuable compounds. In this review, we will briefly describe the enzymes that PLP participated and focus on the advances in vitamin B 6 biosynthesis and discuss its application to engineering bacteria that overproduce other commercially valuable substances including cadaverine, 3,4‐dihydroxyphenylacetate‐ l ‐alanine, γ‐aminobutyric acid, and l ‐phosphinothricin. It will provide a reference for the biosynthesis of vitamin B 6 or other valuable compounds in which PLP participates in the reaction, and we also look forward to the future development prospect of PLP.