Validation and Clinical Application of a Volumetric Absorptive Microsampling Method for 14 Psychiatric Drugs
Mike E. Stern, Melanie Giebels, Tilman Fey, Margit Lübking, Judith Alferink, Georg Hempel
Abstract
Aim: Volumetric absorptive microsampling offers a hematocrit insensitive way for microsampling. The aim of this study was to develop a method for routine healthcare for 14 antidepressants, antipsychotics and their active metabolites on a single-quadrupole HPLC–MS. A clinical validation study to determine conversion factors from capillary blood to plasma concentration was conducted afterward. Results: The method was validated according to current guidelines except for one substance. Five substances were measured in 49 patient samples and conversion factors could be derived with Passing–Bablok and Bland–Altman analysis. Conclusion: A reliable extraction and analysis method for commonly used antidepressants and antipsychotics was developed and validated. The method with the obtained conversion factors can now be used in routine healthcare.