Enantiospecific Detection of D‐Amino Acid through Synergistic Upconversion Energy Transfer
Yongan Tang, Xiaoyan Zhong, Shuangqian Yan, Xiaowang Liu, Xiaowang Liu, Liang Cheng, Yu Wang, Xiaogang Liu, Xiaogang Liu
Abstract
D-amino acids (DAAs) are indispensable in regulating diverse metabolic pathways. Selective and sensitive detection of DAAs is crucial for understanding the complexity of metabolic processes and managing associated diseases. However, current DAA detection strategies mainly rely on bulky instrumentation or electrochemical probes, limiting their cellular and animal applications. Here we report an enzyme-coupled nanoprobe that can detect enantiospecific DAAs through synergistic energy transfer. This nanoprobe offers near-infrared upconversion capability, a wide dynamic detection range, and a detection limit of 2.2 μM, providing a versatile platform for in vivo noninvasive detection of DAAs with high enantioselectivity. These results potentially allow real-time monitoring of biomolecular handedness in living animals, as well as developing antipsychotic treatment strategies.