A <scp>Mitochondria‐Specific</scp> Orange/<scp>Near‐Infrared‐Emissive</scp> Fluorescent Probe for <scp>Dual‐Imaging</scp> of Viscosity and <scp>H<sub>2</sub>O<sub>2</sub></scp> in Inflammation and Tumor Models
Li Fan, Qi Zan, Xiaodong Wang, Shuohang Wang, Yuewei Zhang, Wenjuan Dong, Shaomin Shuang, Chuan Dong
Abstract
Main observation and conclusion Elucidating the intrinsic relationship between viscosity/H 2 O 2 and mitochondria‐associated diseases remains a great challenge owing to the lack of research on multiple diseases models, such as inflammation and malignant tumor models. In this work, we have developed a mitochondria‐specific orange/near‐infrared‐emissive fluorescent probe TTPB, for dual‐imaging of viscosity and H 2 O 2 levels in two different channels. The probe exhibited a remarkable response to viscosity with NIR emission round 666 nm, and was highly sensitive to H 2 O 2 in orange channel with emission peak at 586 nm. Moreover, TTPB has good mitochondria‐specific ability and permits individual detecting of viscosity in NIR channels and H 2 O 2 levels in orange channel in living cells. More notably, TTPB was successfully applied to simultaneously image the viscosity and H 2 O 2 levels in inflammation and cancer models.