PAMAM–PMAA brush-functionalized magnetic composite nanospheres: a smart nanoprobe with tunable selectivity for effective enrichment of mono-, multi-, or global phosphopeptides
Lingzhu Yu, Bin Luo, Zhiyu Li, Jia He, Fang Lan, Yao Wu
Abstract
), excellent selectivity (1 : 500 molar ratios of β-casein/BSA) and high recyclability (five cycles) towards phosphopeptides. Additionally, based on the tunable bonding ability of the nanospheres towards phosphopeptides with different phosphorylation sites, selective enrichment of mono-, multi- or global phosphopeptides was realized by modulating buffer polarity and acidity, making them ideal new nanoprobes for comprehensive phosphoproteome analysis.
Topics & Concepts
NanoprobeSelectivityBrushComposite numberMaterials scienceChemical engineeringNanotechnologyChemistryComposite materialNanoparticleOrganic chemistryCatalysisEngineeringAdvanced biosensing and bioanalysis techniquesAdvanced Proteomics Techniques and ApplicationsChemical Synthesis and Analysis