Cycloheximide (CHX) Chase Assay to Examine Protein Half-life
Ying Miao, Qian Du, Hong Zhang, Yukang Yuan, Yibo Zuo, Hui Zheng
Abstract
that acts as fungicide. As a ribosome inhibitor, CHX can restrict the translation elongation of eukaryotic protein synthesis. Once protein synthesis is inhibited by CHX, the level of intracellular proteins decreases by degradation through the proteasome or lysosome system. Thus, the CHX chase assay is widely recognized and used to observe intracellular protein degradation and to determine the half-life of a given protein in eukaryotes. Here, we present a complete experimental procedure of the CHX chase assay. Graphical overview.
Topics & Concepts
CycloheximideProtein biosynthesisIntracellularProtein degradationProtein synthesis inhibitorRibosomeCell biologyBiochemistryProteasomeTranslation (biology)ChemistryBiologyRNAMessenger RNAGeneUbiquitin and proteasome pathwaysProtein Degradation and InhibitorsPeptidase Inhibition and Analysis