Endogenous HMGB1 regulates GSDME-mediated pyroptosis via ROS/ERK1/2/caspase-3/GSDME signaling in neuroblastoma.
Chen-Ying Fan, Fang-Hua Ye, Min Peng, Jiajia Dong, Wenwen Chai, Wenjun Deng, Hui Zhang, Liangchun Yang
Abstract
, a ROS agonist) and EGF (an ERK agonist) promoted the cleavage of GSDME and caspase-3 in DDP or VP16 treatment cells, both of which were inhibited by HMGB1 knockdown. Importantly, these data were further supported by the in vivo experiment. Our study suggests that HMGB1 is a novel regulator of pyroptosis via the ROS/ERK1/2/caspase-3/GSDME pathway and a potential drug target for therapeutic interventions in neuroblastoma.
Topics & Concepts
PyroptosisGene knockdownCancer researchHMGB1Programmed cell deathApoptosisCell biologyChemistryBiologyBiochemistryReceptorHeme Oxygenase-1 and Carbon MonoxideNeonatal Health and BiochemistryInflammasome and immune disorders