Fast whole-brain imaging of seizures in zebrafish larvae by two-photon light-sheet microscopy
Giuseppe de Vito, Lapo Turrini, Marie Caroline Müllenbroich, Pietro Ricci, Giuseppe Sancataldo, Giacomo Mazzamuto, Natascia Tiso, Leonardo Sacconi, Duccio Fanelli, Ludovico Silvestri, Francesco Vanzi, Francesco S. Pavone
Abstract
Light-sheet fluorescence microscopy (LSFM) enables real-time whole-brain functional imaging in zebrafish larvae. Conventional one-photon LSFM can however induce undesirable visual stimulation due to the use of visible excitation light. The use of two-photon (2P) excitation, employing near-infrared invisible light, provides unbiased investigation of neuronal circuit dynamics. However, due to the low efficiency of the 2P absorption process, the imaging speed of this technique is typically limited by the signal-to-noise-ratio. Here, we describe a 2P LSFM setup designed for non-invasive imaging that enables quintuplicating state-of-the-art volumetric acquisition rate of the larval zebrafish brain (5 Hz) while keeping low the laser intensity on the specimen. We applied our system to the study of pharmacologically-induced acute seizures, characterizing the spatial-temporal dynamics of pathological activity and describing for the first time the appearance of caudo-rostral ictal waves (CRIWs).