Marker-assisted confirmation of maternal or zygotic origin of embryogenic cultures and regenerated plantlets of two polyembryonic mango cultivars using SSR markers
S. Sajana, Pious Thomas, K. V. Ravishankar, P. Nandeesha, Reju M Kurian, Anuradha Sane, Hima Bindu
Abstract
In this study, attempts were made to confirm the origin of mango (Mangifera indica L.) embryos produced in vitro through somatic embryogenesis by using explants which were presumed as of diverse origin using SSR markers. SSR markers used in the study detected a total number of 148 alleles per locus with the mean number of alleles ranging from 8 alleles (MiKVR–81) to 11 alleles per SSRs (MiIIHR–21478). The expected heterozygosity ranged from 0.92 (MiKVR–81) to 1.00 (MiKVR–71). The polymorphic information content (PIC) value was maximum (0.895) in MiIIHR – 21478 and minimum (0.830) in MiIIHR – 78. In both cv. Vellaikolumban and cv. Olour, the position of barcode for all nucellar embryos was observed to be similar to that of the maternal parent and the barcode of zygotic embryo in vitro were observed to be different from the mother plant with all 16 primers. Dendrogram constructed using SSR marker data by the Neighbour joining method resulted in two main clusters with Vellakolumban and Olour samples in separate clusters. Each cluster was divided into two subclusters consisting embryos produced from the nucellus tissue and the mother plant in one sub cluster and embryos produced from zygotic embryo formed a separate cluster in both cultivars.