Phase transitions and mechanisms of cryoprotection of serum-/xeno-free media based on dextran and dimethyl sulfoxide
Oleksandr Pakhomov, T. M. Gurina, Viktoria Mazaeva, A. L. Polyakova, Bo Deng, Evgeniy I. Legach, Г. А. Божок
Abstract
SO in lower concentrations (0.7 M) than required for serum-containing media (1.4 M), which may decrease the toxicity of serum-/xeno-free media.
Topics & Concepts
Differential scanning calorimetryPolyethylene glycolDimethyl sulfoxideCryopreservationChemistryDextranMannitolEutectic systemCrystallizationPolyvinylpyrrolidoneChromatographyPolymer chemistryChemical engineeringBiophysicsBiochemistryOrganic chemistryBiologyCell biologyPhysicsEngineeringThermodynamicsAlloyEmbryoSperm and Testicular FunctionSeed Germination and PhysiologyReproductive Biology and Fertility