Optimization of AsCas12a for combinatorial genetic screens in human cells
Peter C. DeWeirdt, Kendall R Sanson, Annabel K. Sangree, Mudra Hegde, Ruth E. Hanna, Marissa N. Feeley, Audrey L Griffith, Teng Teng, Samantha Borys, Christine Strand, J. Keith Joung, Benjamin P. Kleinstiver, Xuewen Pan, Alan Huang, John G. Doench
Topics & Concepts
Cas9CRISPRComputational biologyGuide RNAGenome editingSynthetic biologyBiologyComputer scienceGenome engineeringGenomeSubgenomic mRNAGeneticsGeneCRISPR and Genetic EngineeringRNA and protein synthesis mechanismsAdvanced biosensing and bioanalysis techniques