De novo biosynthesis of τ-cadinol in engineered Escherichia coli
Yue Sun, Shaoting Wu, Xiao Fu, Chongde Lai, Daoyi Guo
Abstract
τ-Cadinol is a sesquiterpene that is widely used in perfume, fine chemicals and medicines industry. In this study, we established a biosynthetic pathway for the first time in engineered Escherichia coli for production of τ-cadinol from simple carbon sources. Subsequently, we further improved the τ-cadinol production to 35.9 ± 4.3 mg/L by optimizing biosynthetic pathway and overproduction of rate-limiting enzyme IdI. Finally, the titer was increased to 133.5 ± 11.2 mg/L with a two-phase organic overlay-culture medium system. This study shows an efficient method for the biosynthesis of τ-cadinol in E. coli with the heterologous hybrid MVA pathway.
Topics & Concepts
Escherichia coliIndustrial and production engineeringBiosynthesisHeterologousOverproductionLimitingEnzymeChemistryBiologyBiochemistryGeneElectrical engineeringEngineeringMechanical engineeringPlant biochemistry and biosynthesisMicrobial Metabolic Engineering and BioproductionAlgal biology and biofuel production