Plastisphere in an Antarctic environment: A microcosm approach

Abstract

Microplastics are present even in remote regions like the Southern Ocean. Once in the water, they are rapidly colonised by marine microorganisms, forming the plastisphere . To address this issue in Antarctic waters, we conducted a microcosm experiment by incubating polypropylene, polyethylene, polystyrene microplastic pellets, and quartz for 33 days on Livingston Island, South Shetland Islands, Antarctica. We analysed plastic colonisation and plastisphere dynamics using scanning electron microscopy, flow cytometry, bacterial cultivation, qPCR, and 16S rRNA gene metabarcoding. Our results show rapid and consistent colonisation, although biomass formation was slightly slower than in other oceans, indicating unique environmental constraints. Time was the main factor influencing biofilm communities, while plastic polymer types had little effect. We observed a transition in microbial communities from early- to late-biofilm stages between days 12 and 19. Additionally, we described the bacterial plastisphere composition in this Antarctic environment, including the presence of hydrocarbon-degrading bacteria. • Colonisation of plastics in the Antarctic is slower than in other regions. • Time was the main driver of biofilm community development. • Plastic polymers did not significantly influence the bacterial community. • Potential plastic-degrading bacteria were detected, but no pathogenic strains. • Multiple analytical approaches are needed to understand plastisphere colonisation.

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