Antitumor effects of hyperthermia with plasma‐treated solutions on 3D bladder tumor spheroids
Hao Zhang, Jishen Zhang, Shengduo Xu, Zifeng Wang, Dehui Xu, Li Guo, Dingxin Liu, Michael G. Kong, Mingzhe Rong
Abstract
Abstract As a form of cold atmospheric plasma application, plasma‐treated solutions have shown promise in tumor therapy. Especially for the treatment of intracavitary tumors, plasma‐treated solutions combined with traditional perfusion therapy have strong application potential. Here, we assessed the antitumor effects of the plasma‐treated medium combined with hyperthermia on three‐dimensional bladder tumor spheroids in vitro. We found that the combined treatment of plasma‐treated medium and hyperthermia (43°C) presented better antitumor effects than a single treatment. Further research showed that synchronous hyperthermia can promote the penetration of plasma‐generated reactive oxygen species from the plasma‐treated medium into cancer cells. Our results suggest that hyperthermic perfusion therapy using plasma‐treated solutions may serve as a novel and valuable therapeutic strategy for bladder cancer.