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A trimodular bacterial enzyme combining hydrolytic activity with oxidative glycosidic bond cleavage efficiently degrades chitin

Sophanit Mekasha, Tina R. Tuveng, Fatemeh Askarian, Swati Choudhary, Claudia Schmidt‐Dannert, Axel Niebisch, Jan Modregger, Gustav Vaaje‐Kolstad, Vincent G. H. Eijsink

2020Journal of Biological Chemistry39 citationsDOIOpen Access PDF

Abstract

1381 activity requires close spatial proximity of the LPMO10 and the Chi18 domains. The demonstration of intramolecular synergy between LPMOs and hydrolytic enzymes reported here opens new avenues toward the development of efficient catalysts for biomass conversion.

Topics & Concepts

ChitinGlycosidic bondChitinaseGlycoside hydrolasePolysaccharideEnzymeBiochemistryHydrolysisChemistryBiologyChitosanStudies on Chitinases and ChitosanasesEnzyme Production and CharacterizationBiofuel production and bioconversion
A trimodular bacterial enzyme combining hydrolytic activity with oxidative glycosidic bond cleavage efficiently degrades chitin | Litcius