Optimization of electrospun CQDs-Fe3O4-RE loaded PVA-cellulose nanofibrils via central composite design for wound dressing applications: Kinetics and in vitro release study
Seyed Hesamodin Rooholghodos, Mehrab Pourmadadi, Fatemeh Yazdian, Hamid Rashedi
Abstract
Wound skin infections can cause significant morbidity and even mortality. Cellulose nanofibrils (CNFs) are a type of nano cellulose that have reached notable attention due to their inimitable properties. In this study, in order to prepare a novel wound dressing , CNFs are composited with poly (vinyl alcohol) (PVA) to enhance mechanical properties and increase cell proliferation and migration. Also, carbon quantum dots (CQDs)- Fe 3 O 4 was introduced as a novel antibacterial, and rosemary extract (RE) was composited with this to reduce its cell toxicity. PVA - CNFs/ CQDs- Fe 3 O 4 - RE nanofiber was prepared using the electrospinning method . Then, to maximize tensile strength , total elongation, and percentage swelling of PVA - CNFs/ CQDs- Fe 3 O 4 - RE electrospun nanofiber , parameters of crosslinking duration and the concentration of CQDs- Fe 3 O 4 -RE were optimized employing central composite design , and optimized electrospun nanofiber (OEN) as a novel wound dressing was prepared. Results exhibited, the high antibacterial properties of CQDs-Fe 3 O 4 -RE. Also, CNFs and CQDs- Fe 3 O 4 -RE increased the tensile strength of OEN. Moreover, CNFs and RE reduce wound area percentages and increase the percentage of cell viability , respectively. Therefore, OEN was introduced as a suitable wound dressing due to its appropriate surface roughness, mechanical properties, WVTR , biodegradation, prolonged release, non-toxicity, and high cell proliferation and migration ability.