One-step knock-in of two antimicrobial peptide transgenes at multiple loci of catfish by CRISPR/Cas9-mediated multiplex genome engineering
Jinhai Wang, Indira Medina Torres, Mei Shang, Jacob Al-Armanazi, Hamza Dilawar, Darshika Hettiarachchi, Abel Paladines-Parrales, Barrett Chambers, Kate B. Pottle, Misha Soman, Baofeng Su, Rex A. Dunham
Topics & Concepts
CRISPRBiologyCas9Genome editingTransgenemCherryGeneGeneticsMolecular biologyTransgenesisMutagenesisMutationGreen fluorescent proteinEmbryogenesisReproductive biologyCRISPR and Genetic EngineeringRNA Interference and Gene DeliveryAdvanced biosensing and bioanalysis techniques