The effect of niosome preparation methods in encapsulating 5-fluorouracil and real time cell assay against HCT-116 colon cancer cell line
Onyinyechi Lydia Ugorji, Ogochukwu Ngozi Chidimma Umeh, Chukwuma O. Agubata, Dickson Adah, Nicholas C. Obitte, Amarauche Chukwu
Abstract
, indicating strong hydrogen bonds. The RTCA showed 5-fluorouracil-loaded niosomes caused more sustained cell death compared to the pure drug and blank niosomes. The methods of preparation affected the particle size, polydispersity index, entrapment efficiency, and the physical stability of the vesicles. The thin film hydration method was more robust in the entrapped 5-fluorouracil and showed lower particle sizes when compared to all the other methods. RTCA showed sustained cell death in real time.
Topics & Concepts
NiosomeDispersityParticle sizeSonicationFourier transform infrared spectroscopyCytotoxicityChemistryChromatographyZeta potentialDynamic light scatteringMaterials scienceNanoparticleVesicleIn vitroChemical engineeringNanotechnologyMembraneOrganic chemistryBiochemistryPhysical chemistryEngineeringAdvancements in Transdermal Drug DeliveryAdvanced Drug Delivery SystemsDermatology and Skin Diseases