The establishment of multiple knockout mutants of Colletotrichum orbiculare by CRISPR-Cas9 and Cre-loxP systems
Kohji Yamada, Toya Yamamoto, Kanon Uwasa, Keishi Osakabe, Yoshitaka Takano
Topics & Concepts
BiologyCRISPRGene knockoutGene targetingCas9GeneMutantVirulenceComputational biologyGeneticsCre recombinaseCre-Lox recombinationCRISPR interferenceTransgeneGenetically modified mousePlant tissue culture and regenerationCRISPR and Genetic EngineeringPlant-Microbe Interactions and Immunity