The immunoenhancement effects of starfish<i>Asterias rollestoni</i>polysaccharides in macrophages and cyclophosphamide-induced immunosuppression mouse models
Yingjuan Liu, Xiaolin Wu, Yue Wang, Weihua Jin, Yunliang Guo
Abstract
. We aimed to detect the immunoenhancement effects of SF-2 in macrophages and cyclophosphamide (CYP)-induced immunosuppression mouse models. RAW 264.7 macrophage cells were treated with SF-2 for different periods of time (0 h, 0.5 h, 1 h, 3 h, 6 h, and 9 h) and the results showed that SF-2 promoted the production of nitric oxide and up-regulated the levels of pro-inflammatory cytokines and related proteins, such as TNF-α, IL-1β, IL-6, COX-2, MMP-9, and iNOS in a time-dependent manner. In addition, SF-2 activated NLRP3 inflammasome and the MAPK/NF-κB signaling pathway, thus promoting its immunoenhancement effects. Moreover, we co-cultured the primary peritoneal macrophages with SF-2 for 6 h and found that SF-2 enhanced the expression of NLRP3 inflammasome and the release of cytokines. Furthermore, SF-2 significantly increased the body weight, spleen index, thymus index, and inflammatory cell counts in CYP-induced immunosuppression mouse models. These results indicate that SF-2 is a potential immunoenhancement mediator that acts by activating the NLRP3 inflammasome and MAPK/NF-κB pathway.