Zika virus lateral flow assays using reverse transcription-loop-mediated isothermal amplification
Gna Ahn, SeonHyung Lee, Se Hee Lee, Yun Hee Baek, Min‐Suk Song, Yang‐Hoon Kim, Ji‐Young Ahn
Abstract
-ZIKV LAMP probe, we could demonstrate the purpose and utility of primary and secondary antibodies. Owing to LFA, the resultant ZIKV RT-LAMP products were rapidly and simply assayed in less than 5 min. Further, no preparation step was required to achieve LAMP-probe hybridization, highlighting the utility of this method for field-ready ZIKV diagnosis. Collectively, our findings suggest that ZIKV RT-LAMP combined with LFA could serve as a rapid, accurate, and independent point-of-care detection method for preventing ZIKV outbreaks.
Topics & Concepts
Reverse Transcription Loop-mediated Isothermal AmplificationZika virusLoop-mediated isothermal amplificationLoop (graph theory)VirologyChemistryReverse transcriptaseTranscription (linguistics)Molecular biologyCell biologyBiologyPhysicsVirusPolymerase chain reactionMathematicsBiochemistryGeneLinguisticsDNAPhilosophyCombinatoricsMosquito-borne diseases and controlBiosensors and Analytical DetectionViral Infections and Vectors