Bactericidal and Antioxidant Potential of <i>Moringa oleifera</i> Capped Silver Nanoparticles under Varied Conditions
Fareha Ali, Shaukat Ali, Sana Shahbaz, Muhammad Summer, Nazish Mazhar Ali, Muhammad Adeel Farooq, Tahreem Tanveer, Ali Hassan, Hafiz Abdullah Shakir
Abstract
Abstract Microbial infections have consistently been identified as one of the major threats to global health. In this regard, the current study was designed to synthesize and evaluate the antibacterial activity of Mo‐AgNPs at various concentrations (2, 4, and 8 mg/mL), temperatures (4 °C, 25 °C, 37 °C, and 85 °C) and pH (4, 7, and 11) against Gram‐negative bacteria ( Escherichia coli, Pseudomonas aeruginosa , Klebsiella pneumoniae , Aeromonas veronii ) and Gram‐positive bacteria ( Staphylococcus aureus, Bacillus subtilis ) by the well diffusion method as well as antioxidant potential using DPPH assay at 2 mg/mL and 4 mg/mL. Fabrication of Mo‐AgNPs was authenticated by UV–visible spectroscopy (UV–Vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM), and X‐ray diffraction (XRD). Mo‐AgNPs produced a significant zone of growth inhibition (21.0±0.3 mm) against B. subtilis at 8 mg/mL. At 25 °C, a significant zone of growth inhibition (21.0±0.8 mm) was measured against E. coli . Similarly, at pH 4, Mo‐AgNPs showed significantly highest growth inhibition zones of 12.0±7.5 mm, 12.0±6.3 mm, and 12.0±9.8 mm against P. aeruginosa, K. pneumoniae , and A. veronii , respectively. The present study results indicated that Mo‐AgNPs are stable under varied conditions. Significant free radical scavenging activity (82±0.5 %) of Mo‐AgNPs at 4 mg/mL was observed, which revealed the antioxidant potential of the Mo‐AgNPs.