Rapid Screening of 352 Pesticide Residues in Chrysanthemum Flower by Gas Chromatography Coupled to Quadrupole‐Orbitrap Mass Spectrometry with Sin‐QuEChERS Nanocolumn Extraction
Yuanyuan Wang, Zhijuan Meng, Chun-Yan Su, Sufang Fan, Yan Li, Haiye Liu, Xuan Zhang, Pingping Chen, Yunyun Geng, Qiang Li
Abstract
To analyze pesticide residues, GC coupled with quadrupole‐Orbitrap MS (GC‐Orbitrap‐MS) has become a powerful tool because of its unique characteristics of accurate mass full‐spectrum acquisition, high resolution, fast acquisition rates, and overcoming matrix interference. This paper presents an efficiency evaluation of GC‐Orbitrap‐MS for identification and quantitation in the 352 pesticide residues analysis of chrysanthemum flowers in full‐scan mode. A streamlined pretreatment approach using one‐step extraction and dilution was used, which provided high‐throughput processing and excellent recovery. The samples were extracted using acetonitrile. The extracted solution was purified by a Sin‐QuEChERS Nano column to suppress the matrix in chrysanthemum flowers and determined by GC‐Orbitrap‐MS. The calibration curves for the 352 pesticides obtained by GC‐Orbitrap‐MS were linear in the range of 0.5–200 μ g·kg −1 , with the correlation coefficients higher than 0.99. The limits of detection (LODs) and the limits of quantification (LOQs) for the 352 pesticide residues were 0.3–3.0 μ g·kg −1 and 1.0–10.0 μ g·kg −1 , respectively. The average recoveries in chrysanthemum flower at three levels were 95.2%, 88.6%, and 95.7%, respectively, with relative standard deviations (RSDs) of 7.1%, 7.5%, and 7.2%, respectively. Lastly, the validated method and retrospective analysis was applied to a total of 200 chrysanthemum flower samples bought in local pharmacies. The proposed method can simultaneously detect multipesticide residues with a good performance in qualitative and quantitative detection.