Azole reagents enabled ligation of peptide acyl pyrazoles for chemical protein synthesis
Peisi Liao, Chunmao He
Abstract
an N-to-C sequential ligation-MFD strategy, the semi-synthesis of the copper protein azurin, and the efficient assembly of a sulfated hirudin variant and the cyclotide kalata B1, all in a one-pot fashion.
Topics & Concepts
Chemical ligationLigationReagentPeptideChemistryCombinatorial chemistryAzoleNative chemical ligationChemical synthesisBiochemistryOrganic chemistryBiologyMolecular biologyMicrobiologyIn vitroAntifungalChemical Synthesis and AnalysisBiochemical and Structural CharacterizationClick Chemistry and Applications