Short and Long-Read Sequencing Survey of the Dynamic Transcriptomes of African Swine Fever Virus and the Host Cells
Ferenc Olasz, Dóra Tombácz, Gábor Torma, Zsolt Csabai, Norbert Moldován, Ákos Dörmő, István Prazsák, István Mészáros, Tibor Magyar, Vivien Tamás, Zoltán Zádori, Zsolt Boldogkői
Abstract
To provide a detailed transcription map about the \ntranscription dynamics of the African Swine Fever Virus, we performed multiplatform \nsequencing using both next-generation short-read sequencing (SRS) and third-generation long-read sequencing (LRS) techniques. The presented \ndataset represent a key resource for studying the ASFV \ntranscriptome at different time points after infection, and of the \neffect of infection on the host gene expression. \nRegarding the SRS approach, the MiSeq instrument \n(Illumina) was used , whereas we applied the MinION portable \nsequencer from Oxford Nanopore Technologies (ONT) for \nfull-length sequencing. The random-primed SRS library was \nrun on a single MiSeq v3 flow cell, whereas three different ONT \nlibraries [direct RNA sequencing (dRNA-Seq), direct cDNA \nsequencing (dcDNA-Seq) and amplified cDNA sequencing) \nwere sequenced on three individual flow cells. Altogether the \nthree LRS experiments resulted in 20,021,413 sequencing reads, of which 139,711 aligned to the viral \ngenome (MN715134.1). The longest average read length was \nobtained using the dcDNA technique (1,299 bp). The average \nlength for the amplified approach ranged between 598 and \n1,017 bp, whereas the dRNA-Seq resulted in an average read \nlength of 953 bp.