Shotgun metagenomic sequencing of the first case of monkeypox virus in Brazil, 2022
Ingra Morales Claro, Camila Malta Romano, Darlan da Silva Cândido, Evelyn Lepka de Lima, José Ângelo Lauletta Lindoso, Mariana Severo Ramundo, Filipe Romero Rebello Moreira, Luiz Alberto Costa Barra, Luciana Marques Sansão Borges, Lucas Alberto Medeiros, Marcia Y. S. Tomishige, Tomas Moutinho, Anderson José Dias da Silva, Camila Cristina Martini Rodrigues, Luiz Cesar Fernandes de Azevedo, Lucy Santos Villas-Boas, Camila Alves Maia da Silva, Thaís Moura Coletti, Erika R. Manuli, Áine O’Toole, Joshua Quick, Nicholas J. Loman, Andrew Rambaut, Nuno R. Faria, Claudia Figueiredo‐Mello, Éster Cerdeira Sabino
Abstract
Monkeypox virus (MPXV), a zoonotic virus endemic to the African continent, has been reported in 33 non-endemic countries since May 2022. We report an almost complete genome of the first confirmed case of MPXV in Brazil. Shotgun metagenomic sequencing was completed in 18 hours, from DNA extraction to consensus sequence generation.