ACtivE: Assembly and CRISPR-Targeted <i>in Vivo</i> Editing for Yeast Genome Engineering Using Minimum Reagents and Time
Koray Malcı, Nestor Jonguitud‐Borrego, Hugo van der Straten Waillet, Urtė Puodžiu̅naitė, Emily J. Johnston, Susan J. Rosser, Leonardo Rios‐Solis
Abstract
DNA assembly methods, restriction enzymes, or extra cloning steps. This study presents a standardizable method to be readily employed to accelerate yeast genome engineering and provides well-defined genomic location alternatives for yeast synthetic biology and metabolic engineering purposes.
Topics & Concepts
CRISPRGenome editingSynthetic biologyGenome engineeringComputational biologyCas9GenomeBiologyPlasmidSaccharomyces cerevisiaeCloning (programming)DNAYeastGeneComputer scienceGeneticsProgramming languageCRISPR and Genetic EngineeringRNA and protein synthesis mechanismsFungal and yeast genetics research