Internal Standard Triggered-Parallel Reaction Monitoring Mass Spectrometry Enables Multiplexed Quantification of Candidate Biomarkers in Plasma
Jacob J. Kennedy, Jeffrey R. Whiteaker, Richard G. Ivey, Aura Burian, Shrabanti Chowdhury, Chia‐Feng Tsai, Tao Liu, Chenwei Lin, Oscar Murillo, Rachel A. Lundeen, Lisa Jones, Philip R. Gafken, Gary Longton, Karin Rodland, Steven J. Skates, John D. Landua, Pei Wang, Michael T. Lewis, Amanda G. Paulovich
Abstract
> 0.999 over 4 orders of magnitude), and sensitivity (median LLOQ < 1 fmol, approximately) to enable rank-ordering of candidate biomarkers for validation studies. Using three plasma pools from breast cancer patients and three control pools, 893 proteins were quantified, of which 162 candidate biomarkers were verified in at least one of the cancer pools and 22 were verified in all three cancer pools. The assay greatly expands capabilities for quantification of large numbers of proteins and is well suited for prioritization of viable candidate biomarkers.