A Sandwich-Type Electrochemical Immunosensor Using Antibody-Conjugated Pt-Doped CdTe QDs as Enzyme-Free Labels for Sensitive HER2 Detection Based on a Magnetic Framework
Hosna Ehzari, Meysam Safari
Abstract
Tumor markers are highly sensitive and play an important role in the early diagnosis of cancer. We developed an electrochemical sandwich-type immunosensor that detects human epidermal growth factor receptor 2 (HER2). Magnetic framework (Fe 3 O 4 @ TMU-24) and AuNPs (Fe 3 O 4 @ TMU-24 -AuNPs) are utilized in this sensing platform. In addition to their high specific surface area and excellent biocompatibility, Fe 3 O 4 @ TMU-24-AuNPs nanocomposites exhibited excellent electrocatalytic properties. The primary antibody of HER2 (Ab 1 ) was immobilized on the surface of the Fe 3 O 4 @ TMU-24-AuNPs. In this sensing method, palatine doped to CdTe QDs (Pt: CdTe QDs) is utilized as a novel labeling signal biomolecule (secondary antibodies). Pt: CdTe QDs own good biocompatibility and excellent catalytic performance. The amperometric technique was used to achieve the quantitative determination of HER2 by using a sandwich-type electrochemical immunosensor. Under the optimum conditions, the dependency of the current signal and HER2 concentration showed a linear region from 1 pg ml −1 –100 ng ml −1 with 0.175 pg ml −1 as the limit of detection. This biosensing device also showed long stability and good reproducibility, which can be used for the quantitative assay of HER2.