Poly-GR repeats associated with ALS/FTD gene <i>C9ORF72</i> impair translation elongation and induce a ribotoxic stress response in neurons
Daoyuan Dong, Zhe Zhang, Yini Li, Malgorzata J. Latallo, Shaopeng Wang, B.Dean Nelson, Rong Wu, Gopinath Krishnan, Fen‐Biao Gao, Bin Wu, Shuying Sun
Abstract
Hexanucleotide repeat expansion in the C9ORF72 gene is the most frequent inherited cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). The expansion results in multiple dipeptide repeat proteins, among which arginine-rich poly-GR proteins are highly toxic to neurons and decrease the rate of protein synthesis. We investigated whether the effect on protein synthesis contributes to neuronal dysfunction and degeneration. We found that the expression of poly-GR proteins inhibited global translation by perturbing translation elongation. In iPSC-differentiated neurons, the translation of transcripts with relatively slow elongation rates was further slowed, and stalled, by poly-GR. Elongation stalling increased ribosome collisions and induced a ribotoxic stress response (RSR) mediated by ZAKα that increased the phosphorylation of the kinase p38 and promoted cell death. Knockdown of ZAKα or pharmacological inhibition of p38 ameliorated poly-GR–induced toxicity and improved the survival of iPSC–derived neurons from patients with C9ORF72 -ALS/FTD. Our findings suggest that targeting the RSR may be neuroprotective in patients with ALS/FTD caused by repeat expansion in C9ORF72 .