Direct Uptake and Intracellular Dissolution of HgS Nanoparticles: Evidence from a Bacterial Biosensor Approach
Yuping Xiang, Yingying Guo, Guangliang Liu, Yanwei Liu, Maoyong Song, Jianbo Shi, Ligang Hu, Yongguang Yin, Yong Cai, Guibin Jiang
Abstract
Mercury sulfide nanoparticles (HgS NPs ), which occur widely in oxic and anoxic environments, can be microbially converted to highly toxic methylmercury or volatile elemental mercury, but it remains challenging to assess their bioavailability. In this study, an Escherichia coli -based whole-cell fluorescent biosensor was developed to explore the bioavailability and microbial activation process of HgS NPs . Results show that HgS NPs (3.17 ± 0.96 nm) trigger a sharp increase in fluorescence intensity of the biosensor, with signal responses almost equal to that of ionic Hg (Hg(II)) within 10 h, indicating high bioavailability of HgS NP . The intracellular total Hg (THg) of cells exposed to HgS NPs (200 μg L –1 ) was 3.52–8.59-folds higher than that of cells exposed to Hg(II) (200 μg L –1 ), suggesting that intracellular HgS NPs were only partially dissolved. Speciation analysis using size-exclusion chromatography (SEC)–inductively coupled plasma mass spectrometry (ICP-MS) revealed that the bacterial filtrate was not responsible for HgS NP dissolution, suggesting that HgS NPs entered cells in nanoparticle form. Combined with fluorescence intensity and intracellular THg analysis, the intracellular HgS NP dissolution ratio was estimated at 22–29%. Overall, our findings highlight the rapid internalization and high intracellular dissolution ratio of HgS NPs by E. coli, and intracellular THg combined with biosensors could provide innovative tools to explore the microbial uptake and dissolution of HgS NPs .