3D Patterning of cells in Magnetic Scaffolds for Tissue Engineering
Vitaly Goranov, Tatiana Shelyakova, Roberto De Santis, Y. Haranava, A. Makhaniok, Antonio Gloria, Anna Tampieri, Alessandro Russo, Elizaveta Kon, Maurilio Marcacci, Luigi Ambrosio, V. Dediu
Abstract
A three dimensional magnetic patterning of two cell types was realised in vitro inside an additive manufactured magnetic scaffold, as a conceptual precursor for the vascularised tissue. The realisation of separate arrangements of vascular and osteoprogenitor cells, labelled with biocompatible magnetic nanoparticles, was established on the opposite sides of the scaffold fibres under the effect of non-homogeneous magnetic gradients and loading magnetic configuration. The magnetisation of the scaffold amplified the guiding effects by an additional trapping of cells due to short range magnetic forces. The mathematical modelling confirmed the strong enhancement of the magnetic gradients and their particular geometrical distribution near the fibres, defining the preferential cell positioning on the micro-scale. The manipulation of cells inside suitably designed magnetic scaffolds represents a unique solution for the assembling of cellular constructs organised in biologically adequate arrangements.