Circulating level of sPD-1 and PD-1 genetic variants are associated with hepatitis B infection and related liver disease progression
Phạm Thị Minh Huyền, Đặng Thị Ngọc Dung, Peter Weiss, Phan Quoc Hoan, Đào Phương Giang, Ngọ Thị Uyên, Nguyễn Văn Tuấn, Ngo Tat Trung, Thirumalaisamy P. Velavan, Lê Hữu Song, Nghiêm Xuân Hoàn
Abstract
BACKGROUND: Programmed cell death-1 (PD-1) variants and circulating level of soluble PD-1 are associated with susceptibility to malignant and infectious disease. This study aimed to examine the association of PD-1.5 and PD-1.9 variants, and plasma sPD-1 level with hepatitis B virus (HBV) infection and disease progression. METHODS: The study cohort consisted of adults infected with HBV (n=513) - stratified by clinical course, including chronic hepatitis B (CHB, n=173), liver cirrhosis (LC, n=134) and hepatocellular carcinoma (HCC, n=206) - and matched healthy controls (HC, n=196). The PD-1.5 (rs2227981 C/T) and PD-1.9 (rs2227982 C/T) genetic variants were genotyped by Sanger sequencing, and plasma sPD-1 levels were quantified by enzyme immunoassay. RESULTS: =0.031]. CONCLUSION: sPD-1 level was associated with liver inflammation and progression of liver fibrosis, and the PD-1.5 and PD-1.9 variants were associated with HBV infection and progression of liver disease.