Genetic engineering in organoids
Isaree Teriyapirom, Andreia S. Batista-Rocha, Bon‐Kyoung Koo
Abstract
Three-dimensional organoids have been widely used for developmental and disease modeling. Organoids are derived from both adult and pluripotent stem cells. Various types are available for mimicking almost all major organs and tissues in the mouse and human. While culture protocols for stepwise differentiation and long-term expansion are well established, methods for genetic manipulation in organoids still need further standardization. In this review, we summarized different methods for organoid genetics and provide the pros and cons of each method for designing an optimal strategy.
Topics & Concepts
Human geneticsOrganoidMolecular medicineComputational biologyBiologyGeneticsGeneCell cyclePluripotent Stem Cells ResearchRenal and related cancersCRISPR and Genetic Engineering