Development of a green simple molecularly imprinted nanoprobe for rapid determination of trace thiamphenicol residual in animal derived foods
Shujuan Chen, Wanlin Ouyang, Yiting Zhu, Jing Han, Yanan Zhang, Likou Zou, Juan Deng, Aiping Liu, Shuliang Liu, Yong Yang
Abstract
An environmentally friendly nitrogen-sulfur-doped carbon quantum dots molecularly imprinted fluorescent probe (N,[email protected]) was prepared for the detection of thiamphenicol (TAP) in animal-derived foods. The N,S-CDs, the core fluorophores in optical sensors, were synthesized from food waste of crayfish shells and l-cysteine and assembled to molecularly imprinted layers by Si–O–Si bond. The fluorescence produced by the novel probe in response to TAP ranged from 10.00 to 300.0 μg/L, with a detection limit of 6.20 μg/L. The developed method was also successfully applied to real milk, fish and tap water with 92.08–108.4% recoveries, which agrees with the high-performance liquid chromatography (HPLC) analysis (89.15%–110.7%). The developed probe is cheap with a short detection time (30 min) and provides a theoretical and practical operation basis for monitoring TAP residue levels.