Survival of <i>Salmonella enterica</i> subsp. enterica serovar Javiana and <i>Listeria monocytogenes</i> is dependent on type of soil‐free microgreen cultivation matrix
G. Misra, Kristen E. Gibson
Abstract
AIMS: This study measured the survival of Listeria monocytogenes and Salmonella enterica subsp. enterica serovar Javiana over a 10-day period on four soil-free cultivation matrix (SFCM) types in the absence of microgreens and fertilizers. METHODS AND RESULTS: CFU per ml bacteria, incubated at room temperature, and analysed on day 0, 1, 3, 6, and 10. Statistically significant differences in pathogen survival were observed across multiple time points for hemp and Biostrate compared to CC, peat and bacteria in phosphate buffered saline (PBS) (P < 0·05). S. Javiana showed greater overall survival compared to Listeria (P < 0·0002). By day 10, S. Javiana persisted at the initial inoculum concentration for hemp and Biostrate while declining by 1-2 log CFU per ml in CC, peat and PBS. Listeria also persisted at the initial concentration in hemp and Biostrate but decreased to 1 log CFU per ml in peat and below the detection limit in CC and PBS. CONCLUSIONS: Overall, there are survival differences between bacterial pathogens in SFCM used in microgreen production systems. To our knowledge, this is the first comparison of survival among SFCM involving a S. enterica serovar and L. monocytogenes, and the first study comparing CC, Biostrate and hemp. SIGNIFICANCE AND IMPACT OF THE STUDY: Microgreens production systems predominantly utilize soil alternatives, and it is not well-understood how pathogen transmission risk may be affected by the type of SFCM. The results of this study impact the microgreen industry as media selection may be used to reduce the risk of bacterial pathogen proliferation and transmission to the plant potentially resulting in potential foodborne illness.