Single-molecule transport kinetics of a glutamate transporter homolog shows static disorder
Didar Ciftci, Gerard H. M. Huysmans, Xiaoyu Wang, Changhao He, Daniel S. Terry, Zhou Zhou, Gabriel A. Fitzgerald, Scott C. Blanchard, Olga Boudker
Abstract
Single-molecule detection of transport turnovers shows a distribution of rates with fast transporters favored by mutations.
Topics & Concepts
Pyrococcus horikoshiiTransporterPeriplasmic spaceFörster resonance energy transferBiophysicsExcitatory amino-acid transporterKineticsNeurotransmitter transporterChemistryAmino acidGlutamate receptorMutantPopulationMembrane transport proteinBiochemistryBiologyFluorescenceGeneEscherichia coliMedicineQuantum mechanicsReceptorPhysicsEnzymeEnvironmental healthNeuroscience and Neuropharmacology ResearchIon channel regulation and functionLipid Membrane Structure and Behavior