A facile strategy for the construction of a phage display cyclic peptide library for the selection of functional macrocycles
Hua Xiang, Li‐Wen Bai, Xindan Zhang, Ting Dan, Peng Cheng, Xiaoqin Yang, Hong‐Lian Ai, Kai Li, Xinxiang Lei
Abstract
-member library and subsequent screening successfully identified cyclic peptide binders targeting three therapeutically relevant proteins: PTP1B, NEK7, and hKeap1. The results confirm the efficacy in rapidly obtaining active ligands with micromolar potency. This work provides a fast and efficient operable high-throughput platform for screening functional peptide macrocycles, which hold promise for broad application in therapeutics, chemically biological probes, and disease diagnosis.
Topics & Concepts
Phage displayCyclic peptideSelection (genetic algorithm)Combinatorial chemistryPeptide libraryPeptideComputational biologyChemistryComputer scienceBiologyBiochemistryArtificial intelligenceGenePeptide sequenceGlycosylation and Glycoproteins ResearchChemical Synthesis and AnalysisCarbohydrate Chemistry and Synthesis