Phase-separated condensate-aided enrichment of biomolecular interactions for high-throughput drug screening in test tubes
Min Zhou, Weiping Li, Jian Li, Leiming Xie, Rongbo Wu, Liang Wang, Shuai Fu, Wei Su, Jianyang Hu, Jing Wang, Pilong Li
Abstract
est tubes (CEBIT). The readout of CEBIT is the selective recruitment of biomolecules into phase-separated condensates harboring their cognate binding partners. We tailored CEBIT to detect various biomolecular interactions and activities of biomolecule-modifying enzymes. Using CEBIT-based high-throughput screening assays, we identified known inhibitors of the p53/MDM2 (MDM2) interaction and of the histone methyltransferase, suppressor of variegation 3-9 homolog 1 (SUV39H1), from a compound library. CEBIT is simple and versatile, and is likely to become a powerful tool for drug discovery and basic biomedical research.
Topics & Concepts
Protein–protein interactionHigh-throughput screeningDrug discoveryComputational biologyBiomoleculeDruggabilityChemistryBiologyBiochemistryGeneRNA Research and SplicingRNA modifications and cancerRNA and protein synthesis mechanisms