Increased Nitrogenase Activity in Solar‐Driven Biohybrids Containing Non‐photosynthetic Bacteria and Conducting Polymers
Yue Zeng, Haotian Bai, Wen Yu, Shengpeng Xia, Qi Shen, Yiming Huang, Fengting Lv, Guillermo C. Bazan, Shu Wang
Abstract
Abstract A conductive polymer‐based photosynthetic biohybrid is constructed to enhance biological nitrogen fixation by increasing nitrogenase activity in the non‐photosynthetic bacterium Azotobacter Chroococcum ( A. Chroococcum ). The light‐harvesting cationic poly(fluorene‐alt‐phenylene) (PFP) electrostatically binds to the surface of the bacteria and possesses satisfactory conductivity to facilitate electron transfer to the bacterium, promoting the nitrogen fixation pathway through redox proteins on the surface of the bacteria when under illumination. Therefore, the nitrogenase activity, hydrogen, NH 4 + ‐N and L‐amino acids production are increased by 260 %, 37 %, 44 %, and 47 %, respectively. The expression levels of nif D and nif K encoding molybdenum‐iron (MoFe) protein and relevant nitrogen‐fixing proteins are up‐regulated. These photoactive conductive polymer‐bacteria biohybrids provide a new method for improving the biological nitrogen fixation capability of non‐photosynthetic nitrogen‐fixing bacteria.