A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions
Dae‐Kyum Kim, Jennifer J. Knapp, Da Kuang, Aditya Chawla, Patricia Cassonnet, Hunsang Lee, Dayag Sheykhkarimli, Payman Samavarchi‐Tehrani, Hala Abdouni, Ashyad Rayhan, Roujia Li, Oxana Pogoutse, Étienne Coyaud, Sylvie van der Werf, Caroline Demeret, Anne‐Claude Gingras, Mikko Taipale, Brian Raught, Yves Jacob, Frederick P. Roth
Abstract
The world is facing a global pandemic of COVID-19 caused by the SARS-CoV-2 coronavirus. Here we describe a collection of codon-optimized coding sequences for SARS-CoV-2 cloned into Gateway-compatible entry vectors, which enable rapid transfer into a variety of expression and tagging vectors. The collection is freely available. We hope that widespread availability of this SARS-CoV-2 resource will enable many subsequent molecular studies to better understand the viral life cycle and how to block it.