Spatial analysis for highly multiplexed imaging data to identify tissue microenvironments
Ellis Patrick, Nicolas Canete, Sourish S. Iyengar, Andrew N. Harman, Greg T. Sutherland, Pengyi Yang
Abstract
Highly multiplexed in situ imaging cytometry assays have made it possible to study the spatial organization of numerous cell types simultaneously. We have addressed the challenge of quantifying complex multi-cellular relationships by proposing a statistical method which clusters local indicators of spatial association. Our approach successfully identifies distinct tissue architectures in datasets generated from three state-of-the-art high-parameter assays demonstrating its value in summarizing the information-rich data generated from these technologies.
Topics & Concepts
Computer scienceSpatial analysisMultiplexingMass cytometryIn situData miningComputational biologyPattern recognition (psychology)Remote sensingArtificial intelligenceBiologyGeographyMeteorologyGenePhenotypeTelecommunicationsBiochemistrySingle-cell and spatial transcriptomicsCell Image Analysis TechniquesGene expression and cancer classification